Research of the effect of 1,25-Dihydroxyvitamin D3 on inflammation in rats with chronic obstruction pulmonary disease

Abstract

Objective To investigate the effect and mechanism of 1,25-Dihydroxy vitamin D3 (1,25 (OH)2 D3 ) on inflammation in chronic obstructive pulmonary disease (COPD). Methods Thirty male Wistar rats were randomly divided into three groups, A: control group, B: COPD group, C:1,25(OH)2D3+COPD group. B and C groups was established by cigarette inhalation and LPS was instilled intratracheally once for every two weeks. 1,25(OH)2D3 was administered intraperitoneally in C group. Bronchoalveolar lavage fluid (BALF) was collected for total and differential cell counts, and the concentrations of IL-8 and tumor necrosis factor alpha (TNF-α) in BALF were detected by ELISA. The levels in serum of CD4, CD8 and the levels in lung of CD11c, CD80 to mark DC were measured by flow cytometer, pathologic changes of airways and lung tissues was observed by hematoxylin and eosin (HE).Results The pathologic changes of airways and lung tissue of B and C groups, but C group were signficanfly light than B group ( P ＜0.05). The 1evels of serum CD4 and CD4/CD8 were lower in B and C groups than that in the control group. and IL-8 and TNF-α inserum and BALF total and PMN cell counts were higher in B and C groups than in the control group ( P ＜0.05),and those were lower in C group than in B group ( P ＜0.05). Conclusions 1,25(OH)2 D3 can relieve airway inflammation, diminish inflammatory cell and inflammatory factors in COPD rats, improve immunological function. Key words: Chronic obstructive pulmonary disease; Dendritic cells; 1,25-Dihydroxyvitamin D3; Immunological function; Inflammation