Research of Rickettsia spp. and Borrelia spp. in dogs in Southeast Brazil

Abstract

Dogs are important in the epidemiology of tick-borne diseases, playing a significant role mainly in endemic areas of rickettsiosis and borreliosis, and serving as sentinels in epidemiological surveys. We analyzed the distribution of Rickettsia and Borrelia spp. in dogs and their ticks in the municipalities of Guaxupé, Minas Gerais, and Tapiratiba, São Paulo, Brazil, two areas non-endemic for Baggio-Yoshinari Syndrome (BYS) and rickettsiosis. Serum from 242 dogs of different ages (>12months) and breeds were tested by the Indirect Immunofluorescence Assay (IFA) using specific antigens for Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali, and Rickettsia bellii, and by an indirect Enzyme Linked Immunosorbent Assay (ELISA) for the detection of homologous IgG antibodies against Borrelia burgdorferi American strain G39/40. Ticks were collected from the animals and subjected to PCR and nested PCR for detection of Rickettsia spp. (synthase citrate gene) and Borrelia spp. (flagellin gene). Serological data showed that 7.85% (19/242) of the analyzed dogs were seropositive for at least one of the five Rickettsia antigens tested; one (0.41%) was considered seropositive for R. parkeri (1:64) and one (0.41%) for R. rickettsii (1:256). Nine (3.72%) were considered seropositive for R. bellii and one (0.41%) for R. amblyommatis; a seroprevalence of 13.64% (33/242) for IgG class immunoreactive antibodies against B. burgdorferi was observed. A total of 148 ticks were collected from the dogs; among these 3.40% were identified as larvae of the genus Rhipicephalus and 0.70% of the genus Amblyomma; 89.8% were identified as Rhipicephalus sanguineus, 4.70% Amblyomma sculptum and 1.40% Amblyomma ovale; all negative for PCR of Rickettsia spp. and nested PCR for Borrelia spp.. The serological findings of this study suggest the circulation of Rickettsiae associated with the spotted fever group and vector ticks, just like Borrelia spp. in a non-endemic Brazilian area, drawing attention to the possibility of a zoonotic cycle in the region.