Research of Qualitative ELISA and GC-MS for Atrazine in Soil

Abstract

The qualitative ELISA was used for screening out atrazine in soil and positive results were proved with GC-MS. The recoveries 、Precision and the detection limits were estimated in this paper. The average recoveries of ELISA were 96.64%~98.06%,and coefficient of variations were 1.69%~3.36%,the detection limit was 0.04μg/kg, while The average recoveries of GC-MS were 85.78%~87.46%,and coefficient of variations were 4.3%~6.56%,the detection limit was 0.03μg/kg, when the concentrations of 0.5,1.0,5.0μg/kg atrazine standard were add to soil samples. Then three positive samples that filtered by ELISA were proved with GC-MS, and the results were coherent between two methods. The assay found ELISA is a rapid and exact method with good reproducibility for atrazine determination in soil .