Abstract
Hollow fibre cell fishing with HPLC (HFCF-HPLC) based on the human ovarian cancer cell line SKOV-3, human renal tubular cell line ACHN or hepatoma cell line HepG-2 was employed to screen active groups of coumarin and volatile oil in Radix angelicae sinensis, Radix angelicae dahuricae and Fructus citri sarcodactylis. Simultaneously, hollow fibre liquid phase microextraction with HPLC (HFLPME-HPLC) was conducted to enrich and determine the contents of active components in the same sample solution. Before application, for HFCF-HPLC, cells growth states and survival rates on the fibre, effect of ethanol concentration in the extract of samples on cell survival rates, non-specific binding between fibre active centres and the target components, positive and negative controls and repeatabilities were validated; for HFLPME, extraction solvent, sample phase pH, agitation speed, extraction time and sample phase volume were investigated. Many active components were screened from three medicines. Some of them, such as scoparone, psoralen, bergapten, oxypeucedanin, imperatorin, ligustilide, were identified by MS. The target fishing factors of active components and the cell apoptosis rates of three cells under the medicines effect were researched. The binding sites of active groups on HepG-2 cells were preliminarily determined. The results demonstrated that HFCF-HPLC, coupled with HFLPME-HPLC, is a simple and universal approach to find bioactive components at the cellular level, determine their content and research traditional Chinese medicines (TCMs) entirety effect of multi-component and multi-target. The approach may provide us a new and good solution to clarify the material basis of anti-cancer effect and conduct personalized quality control for the components associated with efficacy in TCMs.
Highlights
Traditional Chinese medicines (TCMs) of Radix angelicae sinensis, Radix angelicae dahuricae and Fructus citri sarcodactylis mainly contain coumarin and volatile oil
The results demonstrated that HFCF-HPLC, coupled with Hollow fibre liquid phase microextraction (HFLPME)-HPLC, is a simple and universal approach to find bioactive components at the cellular level, determine their content and research traditional Chinese medicines (TCMs) entirety effect of multi-component and multi-target
The approach may provide us a new and good solution to clarify the material basis of anti-cancer effect and conduct personalized quality control for the components associated with efficacy in TCMs
Summary
Traditional Chinese medicines (TCMs) of Radix angelicae sinensis, Radix angelicae dahuricae and Fructus citri sarcodactylis mainly contain coumarin and volatile oil. For instance, psoralen, bergapten, isobergapten, oxypeucedanin, imperatorin and isoimperatorin have anti-oxidant [1] and anti-cancer [1]-[3] effects. Volatile oil, such as ligustilide has the properties of anti-cancer [4], anti-platelet aggregation and anti-thrombotic [5]. It is necessary to make clearly the anti-cancer material basis of multicomponent and multi-target synergy effect in three TCMs. Traditional activity screening method [6] is time-consuming with high labor intensity and unlikely to simultaneously screen and quantify active components in the same sample solution. The research of simultaneous screening and quantifying active components in the same sample solution has not been published
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