Research data supporting "TTG1 proteins regulate circadian activity as well as epidermal cell fate and pigmentation"

Abstract

Original data underpinning the figures in the associated publication. Each file is the source data for the paper figure it references. Publication content: The Arabidopsis genome contains three members of the TTG1 (TRANSPARENT TESTA GLABRA 1) WDR subgroup of the WDR family, with very different reported roles. TTG1 is a regulator of epidermal cell differentiation, and of the production of pigments, while LWD1 (LIGHT-REGULATED WD1) and LWD2 (LIGHT-REGULATED WD2) are regulators of the circadian clock. We discovered a new central role for TTG1 WDR proteins as regulators of the circadian system, demonstrated by a lack of detectable circadian rhythms in a triple lwd1lwd2ttg1 mutant. We have demonstrated that there has been subfunctionalisation by protein changes within the angiosperms, with some TTG1 WDR proteins developing a stronger role in circadian clock regulation while losing the protein characteristics essential for pigment production and epidermal cell specification, and others weakening their ability to drive circadian clock regulation. File source_fig2.xlsx is an excel spreadsheet containing leaf pixel position and CCA:luminescence data for triple mutant (lwd1 lwd2 ttg1), wild type and various single or double mutant combinations of these 3 genes, all in Arabidopsis. See figure 2 of main manuscript. File source_fig3.xlsx is an excel spreadsheet containing CCAi:LUC luminescence data for various mutant combinations and wild type Arabidopsis. See figure 3 of main manuscript. File source_fig5.xlsx is an excel spreadsheet containing CCA@LUC luminescence data for wild type, mutant and transgenic lines (expressing the TTG1, LWD1 or LWD2 gene from the CaMV 35S promoter in various mutant backgrounds). See figure 5 of main manuscript. File source_fig6.xlsx is an excel spreadsheet containing CCA:LUC luminescence data for various mutants expressing the Marchantia polymorpha or Amborella trichopoda genes from the TTG family. See figure 6 of main manuscript. source_ED_fig 2. xlsx is an excel spreadsheet showing anthocyanin content in mg/g of dry weight. Comparison between WT, ttg1-1 mutant and ttg-1-1 mutant ectopically expressing TTG1, LWD1, LWD2, MpWDR1, MpWDR2, MpWDR3, AmLWD. See extended data figure 2 of main manuscript. source_ED_fig3.xlsx is an excel spreadsheet providing root hair count in 2.5 mm of the first 5 mm of the root of individuals of the mutant and transgenic genotypes used throughout this study. See extended data figure 3 of main manuscript. source_ED_fig4 is a pdf file showing the original electrophoretic gel images which were combined to make extended data figure 4 of the main manuscript. source_ED_fig5.xlsx is an excel spreadsheet showing number of rosette and cauline leaves at flowering of wild type and mutant and transgenic lines used in this study. See main manuscript extended data figure 5. source_ED_fig6.xlsx is an excel spreadsheet showing CCA:LUC luminescence data for the wild type, transgenic and mutant lines used in this study, alongside expression data of LWD2 in the ttg1lwd1 double mutant compared to WT in three biological replicates, data obtained by qRT-PCR with LWD2 specific primers and reference gene UBQ10. See extended data figure 6 of main manuscript. source_ED_fig7.xlsx is an excel spreadsheet showing trichome numbers on the leaf edge of the ttg1 mutant, double mutants and the triple mutant used in this study. Data represent total trichome number on a plant with 9 leaves. See extended data figure 7 of main manuscript. source_ED_fig8.pdf is a pdf file showing the electrophoretic gels from which extended data figure 8 of the main manuscript was constructed. source_ED_fig9.xlsx is an excel spreadsheet showing rosette and cauline leaf number at flowering of individuals of the wild type, mutant and transgenic genotypes used in this study. See extended data figure 9 of main manuscript.