Research and prospect of CRISPR system in marine biological system

Abstract

Gene editing is a widely used and promising technology in biology field. Its ability to precisely edit the endogenous genes of organisms is a major boost to the research process in the biological sciences. Among these gene editing technologies, CRISPR is a more effective system and it is in a wider range of applications. The CRISPR system has advantages over Zinc finger nuclease (ZFNs) technology and transcription activator-like effector nuclease (TALENs) technology, including higher efficiency, gentler construction and a decreased risk of off-target effects. Currently, the treatment of diseases, the creation of animal models, and the genetic breeding of plants all make extensive use of CRISPR technology. Despite its wide request in model construction, its application in marine organisms is just beginning. This review systematically introduces the classification, development of gene editing technology, and summarizes the current status and development prospects of the application of CRISPR system in marine organisms.