Abstract
Aptamer, as a novel recognition molecule, shows great potential in small molecule sensing analysis and detection. Selection of aptamers for small molecules is a pre-requisite for their application. This paper summarized nearly 100 aptamers which were sequenced against 80 kinds of small molecular targets (e.g. toxins, antibiotics and hormones) from 2015 to 2019. Their equilibrium dissociation constants (KD) and determination methods were also summarized. The development and enhancements of systematic evolution of ligands by exponential enrichment (SELEX) method based on graphene oxide, human genome, quartz crystal microbalance and in silico were briefly introduced.
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