Abstract
Nucleic acid detection plays an important role in environmental monitoring, food safety, pathogen detection, genetic disease detection and so on. At present, nucleic acid detection methods mainly include nucleic acid molecular hybridization and polymerase chain reaction. Nucleic acid detection of Corona Virus Disease 2019 has become an indispensable part of our daily life. With the establishment of new detection methods, nucleic acid detection is developing towards single molecule sensitivity and single base specificity. Colloidal gold method and magnetic particle chemiluminescence method have been widely used in the autonomous detection of COVID-19，while both approaches have their limitations. There is a more efficient and specific approach: CRISPR-Cas13 system
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