Research about cell microarray of Ets1 mRNA and e-Met protein expression in lung cancer

Abstract

Objective To develop cell microarray and apply a rapid,normative,simple and economical method for the scientific research in cell fields and clinical work. Methods An 7 × 16 cell microarray was designed using the home-made cell microarray survey setting （the patent appling No. 200520027814 0）. HE stain,immunol histochemistry,fluorescence in situ hibridization. Results The made-up cell microarray was 112 points, and the diagnosis resulted from the cell microarray was totally same to that from the corresponding spread pieces under the microscope. After FISH stain of Etsl mRNA and IHC of c-Met,the positive expression was totally same to that from the routine spread pieces. Comparing the positive expression rate of Etsl mRNA and IHC of c-Met in the Ⅲ＋Ⅳ stages of lung cancer by the cell microarray to that by the tissue microarray, there was no significant difference between them （ P 〉0.05）. Conclusions Cell microarray is a simple,economical method and has low rate of experimental error. It has advantages of rapid diagnosis, high specificity, and large information. It will be broadly applied in clinical diagnosis, scientific research and epidemiologic screening. Key words: Cell microarray; Cell microarray survey setting; Immunol histochemistry; Fluorescence insitu hibridization