Abstract
Genetic improvement of Cajanus cajan (L.) Millspaugh through gene introgression involving wild species of the genus using conventional methods is hampered due to the prevailing post-zygotic barriers. An in vitro technique has been used for rescuing the hybrid embryos between C. cajan and C.platycarpus, that are otherwise des tined to abort under in vivo conditions. 11 to 15 days old embryos developed into only undifferentiated callus on both MS and B5 medium supplemented with 2,4-D/IAA alone and in combination with Kinetin. On the other hand, 16-20 days old embryos could be success fully rescued on MS/B5 + 1.0 mgL^-1 IAA + 0.5 or 1.0 mgL^-1 Kinetin. Successful embryo rescue operation is largely governed by age of the explant, apart from other decisive factors like hormonal combinations and their concentrations in the culture medium.
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