Abstract
Introduction: Tuberculosis, a disease of antiquity continues to cause high morbidity and mortality. In Sustainable Development Goals and End Tb Strategy proposed by WHO includes Drug Susceptibility Testing as major component. Conventional methods are time consuming and molecular methods are rapid but expensive and demanding. Resazurin assay is a rapid colorimetric redox indicator method of Drug Susceptibility Testing. Materials and Methods: 67 strains of M. tuberculosis isolated from pulmonary tuberculosis patients were tested using indirect absolute concentration method by resazurin assay and compared with drug incorporated Lowenstein – Jensen media for Isoniazid and Rifamycin. Results: There was 91.5 % and 91.7% concordance between both methods for INH and Rifamycin respectively. Resazurin assay for INH was 100% sensitive and 61.54% specific. Sensitivity and specificity for RMP was 98.04% and 62.5% respectively. The duration required for INH was 7.0 ± 2.07 and for RMP 6.58 ± 1.58 days. Conclusions: Resazurin assay is sensitive, rapid, cheap, technically simple drug susceptibility method. Keywords: M. tuberculosis, resazurin assay, drug susceptibility.
Highlights
Tuberculosis, a disease of antiquity continues to cause high morbidity and mortality
Emergence of Multidrug, Total drug resistance among M. tuberculosis is amplifying the global problem. 85% of the disease burden is seen in developing countries of Asia (55%) and Africa (30%)
In this study we have evaluated the Resazurin assay for detection of Rifamycin (RMP) and Isoniazid (INH) resistance among the Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis cases
Summary
Tuberculosis has existed for millennia and still remains a major global health problem. One of the indicator for monitoring implementation of this strategy at global and National levels is 100% coverage of Drug Susceptibility Testing [DST] for tuberculosis patients.[3] DST methods can be divided into slow vs rapid, conventional or growth based vs molecular resistance mutation detection and direct vs indirect. Almar Blue itself is weakly fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin indicating cell viability It is used as an oxidation- reduction indicator in cell viability assays for bacteria, mammalian cells and for measuring aerobic respiration.[5] In this study we have evaluated the Resazurin assay for detection of Rifamycin (RMP) and Isoniazid (INH) resistance among the Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis cases
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