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Abstract
In vitro maturation of ovarian oocytes from normally cycling hamsters was obtained, for the first time under conditions which were completely defined, by adjusting the calculated osmolarity of the medium to 286 mOsmol and by supplying 4 amino acids—isoleucine, glutamine, phenylalanine and methionine. The new chemically defined medium (GH-1) required no macromolecular supplement, although a slightly greater proportion of oocytes matured when polyvinylpyrrolidone was added. A reduction of the pO 2 to 5% was necessary for the oocytes to mature when the oil layer was omitted. These studies show that hamster oocytes differ from those of the mouse, which are relatively resistant to elevated osmolarity and oxygen tension and do not need an exogenous supply of fixed nitrogen for their maturation in vitro.
Published Version
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