Reproductive performance of Zebra Fish (Danio rerio) exposed to palm oil mill eflluent in chronic toxicity

Abstract

Palm Oil Mill Effluent (POME) is potentially harmful to the aquatic environment. POME contains high organic material including COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand) TTS (Total Suspended Solid) and various type of heavy metals. of zebra fish (Danio rerio). Reproductin has an important role in producing new individuals which directly affect the population. Impaired reproductive performance potentially impairs juvenile production optimization. The present study investigated how sub-chronic toxicity of POME impact the reproductive performance used Completely randomized Design (CRD) in three treatments and four replicates based on value of LC50-96 hours (5.156 ml/l): Control (0 ml/L), treatment A 10% POME (0,5 ml/L), treatment B 20 % POME (1 ml/L). The fecundity, relative fecundity, GSI, and egg diameter were analyzed. Data was analyzed with Analysis of Variance (ANOVA) and followed with Least Significance Difference (LSD) test. Results showed that fecundity in treatment A (149 ± 38.70) and treatment B (85± 11.35) were significantly decreased compared to the control (219 ± 42.38) (P<0.05). While relative fecundity significantly decreased in treatment B (0.33 ± 0.13) rather than control (0,87 ± 0,14). Significantly decline is also observed on GSI in tretment A (4.79 ± 2.55%) and treatment B (2.55 ± 0.21%) compared to control (6.96 ± 1.70%). While the egg diameter only shows a significantly decline in treatment B (0.57 ± 0.18 mm) compared to control (0.71 ± 0.27 mm).