Reproductive Pathological Changes Associated with Experimental Subchronic Corynebacterium pseudotuberculosis Infection in Nonpregnant Boer Does.

A M Othman,A W Haron,Y Abba,A A Saharee,M A M Lila,Y M Ilyasu,F F A Jesse,M Zamri-Saad

doi:10.1155/2016/4624509

A M Othman, A W Haron + Show 6 more

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https://doi.org/10.1155/2016/4624509

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Abstract

Corynebacterium pseudotuberculosis causes caseous lymphadenitis (CLA), which is a contagious and chronic disease in sheep and goats. In order to assess the histopathological changes observed in the reproductive organs of nonpregnant does infected with the bacteria, 20 apparently healthy adult Boer does were divided into four inoculation groups, intradermal, intranasal, oral, and control, consisting of five goats each. Excluding the control group, which was unexposed, other does were inoculated with 107 CFU/1 mL of live C. pseudotuberculosis through the various routes stated above. Thirty days after infection, the ovaries, uterus, and iliac lymph nodes were collected for bacterial recovery and molecular detection, as well as histopathological examination. The mean changes in necrosis, congestion, inflammatory cell infiltration, and oedema varied in severity among the ovaries, uterus, and iliac lymph nodes following different inoculation routes. Overall, the intranasal route of inoculation showed more severe (p < 0.05) lesions in all the organs examined. The findings of this study have shown that C. pseudotuberculosis could predispose to infertility resulting from pathological lesions in the uterus and ovaries of does.

Highlights

Corynebacterium pseudotuberculosis is gram-positive, facultative, anaerobic, small curved bacillus [1, 2]
Bacterial isolations from tissues of goats inoculated through the intranasal and oral inoculation routes tested positive for the 16S RNA, while goats’ inoculated intradermally tested Polymerase Chain Reaction (PCR) positive only from bacterial cultures of the iliac lymph node
The present study reports, for the first time, the changes observed in the reproductive organs and associated lymph nodes in nonpregnant does experimentally infected with C. pseudotuberculosis via intradermal, intranasal, and oral routes

Summary

Introduction

Corynebacterium pseudotuberculosis is gram-positive, facultative, anaerobic, small curved bacillus [1, 2]. It is generally regarded as an important animal pathogen causing caseous lymphadenitis (CLA) in sheep and goats. Several publications have reported that CLA can be transmitted through the oral, intradermal, intranasal, and intraperitoneal routes [3,4,5,6,7]. The organism has been reported to cause significant economic losses to farmers due to hide and meat condemnation [8,9,10,11]. Based on the above study, there is a possibility that the bacteria might affect the reproductive organs of the doe and lead to pathological changes

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