Reproductive cycle and gonadal development of the Atlantic sea urchin Arbacia punctulata in the Gulf of Mexico: changes in nutritive phagocytes in relation to gametogenesis

Abstract

ABSTRACTThe annual reproductive cycle comprises steady gametogenic activities that synchronize gonadal maturation and spawning rhythms, which are important for aquatic organisms including marine echinoderms (Echinodermata, Echinoidea). In this study, we report the annual reproductive cycle, gonadal development, and changes in nutritive phagocytes (NPs, which accumulate nutrients in germ cells) in relation to gametogenesis of the Atlantic sea urchin (Arbacia punctulata, an edible echinoid) in the Gulf of Mexico. Monthly changes in gonadal development and maturation were observed morphologically and histologically. We calculated gonadosomatic index (GSI) and compared the stages of gonadal development in order to determine the NPs index, and characteristics of germ cells (eggs and sperm) during the annual reproductive cycle. According to GSI and histological analyses, gametogenic activities were classified into four stages of both sexes: mature (June–August), spent (September–November), recovery (December–March), and growing (April–May). The GSI values in both sexes were high during summer months. In males, testicular lobules were densely packed with sperm from June to August. In females, however, mature eggs first appeared in some ovaries in May, numerically increased from June to July, and decreased in August. During gametogenesis, on the other hand, NPs in both testes and ovaries were depleted from June to August. Collectively, our results suggest that the Atlantic sea urchin spawns during summer months in the Gulf of Mexico. This is the first report, to the best of our knowledge, on gonadal development and changes in NPs during the annual reproductive cycle of any Arbacia species in the Gulf of Mexico.