Abstract

AbstractReproductive biology (pollen-ovule ratio, pollen viability, germination in vitro pollination and stigma receptivity in vivo) and karyotype characterization by classical and molecular techniques were performed in Passiflora sublanceolata. The pollen-ovule ratio was 83.9, suggesting that this species is facultative autogamous. Pollen viability was below 70% during all anthesis period (6:00 a.m. to 12:00 p.m.). Low in vitro germination rates were observed after anthesis beginning, with none percentage at one, four and six hours and medium percentage (52.9%) at five hour. The stigma remained receptive during the whole anthesis (100%). The average fertilization percentages resulting from controlled pollinations varied between 8.4% at six hour after anthesis beginning and 50% at two and three hour. The fruit characteristics did not show significant differences by the effects of pollination time. The chromosome number was 2n = 22. The average chromosome length and the haploid chromosome length were 1.60 μm and 17.61 μm, respectively. Six CMA

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