Reproducibility of Dietary Biomarkers in a Multiethnic Asian Population.

Abstract

Dietary biomarkers allow for study of diet and disease risk relationships, but a key requirement is that these biomarkers are reproducible and reflect long-term diet. This study assesses reproducibility of selected dietary biomarkers in a multi-ethnic Asian population, and quantifies diet-disease relationship attenuation arising from use of a single biomarker measurement. Intra-class correlation coefficients (ICC) are used to evaluate the reproducibility of urinary isoflavone and enterolignan, total plasma fatty acid (FA), and serum carotenoid concentrations measured 4 months apart in adult Singapore residents (ethnic Chinese, n 59; Malay, n 46; Indian, n 56). Total carotenoid ICC is 0.75 (95% confidence interval (CI) 0.68, 0.81), ranging from 0.63 to 0.84 for individual carotenoids. FA ICC (median) is 0.74 (inter-quartile range 0.70-0.78). Total isoflavone ICC (95% CI) is 0.21 (0.06-0.35). Total enterolignan ICC is 0.42 (0.28, 0.54). Attenuation factors associated with a single time point measure ranged from 0.74 to 0.94 for carotenoids and FAs, and 0.42 to 0.70 for isoflavones and enterolignans. In a multi-ethnic Asian population, single measures of most serum carotenoids and plasma FAs likely represent habitual diet, whereas reproducibility of urinary isoflavones and enterolignans is moderate, possibly due to rapid excretion.