Abstract
Background and ObjectivesThe aims of the first workshop of the International Platelet Immunology Working Party, Asia Regional (IPIWP‐AR), were to identify antibodies against human platelet antigen (HPA), to compare laboratory performance in the detection of the antibodies and to help laboratories to audit and standardize the own in‐house platelet DNA genotyping methods.Materials and MethodsFourteen laboratories from eight countries participated, which were provided with six DNA samples for genotyping, six serum samples, monoclonal antibodies for monoclonal antibody immobilization of platelet antigens (MAIPA) and the mixed passive hemagglutination (MPHA) kits for antibody detection.ResultsMost of the laboratories could detect the anti‐HPA‐2b, anti‐HPA‐4b, anti‐HPA‐5b and Naka antibodies, by the combination of MAIPA and MPHA. However, some had difficulties with the HPA‐1a antibody, possibly dependent on the rarity of HPA‐1b alloantigen among Asian. Although a good concordance was obtained in genotyping, a significant inter‐laboratory variation was observed, possibly dependent on the methods used.ConclusionsThe IPIWP‐AR and the workshop were successfully established, and most laboratories were able to identify majority of HPA antibodies and genotype HPA correctly.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
