Abstract
To the Editor:Drs Kontis and Nayak1Kontis K.J. Nayak N. Hycor manufactures truly quantitative allergen-specific IgE assays.J Allergy Clin Immunol. 2005; 115: 639Abstract Full Text Full Text PDF PubMed Scopus (1) Google Scholar are correct in indicating that our review2Hamilton R.G. Adkinson Jr., N.F. In vitro assays for the diagnosis of IgE mediated disorders.J Allergy Clin Immunol. 2004; 114: 213-225Abstract Full Text Full Text PDF PubMed Scopus (150) Google Scholar did not highlight the Hycor (Hycor Stratagene, Garden Grove, Calif) IgE antibody assay methods as being automated and quantitative “third-generation assays.” Our conclusions were based on the lack of any quantitative IgE antibody data being reported for the Hycor assays to the College of American Pathologists' Diagnostic Allergy (SE) Survey. Since the SE survey is the most widely subscribed independent diagnostic allergy proficiency survey in North America, we believe that it accurately reflects the state of allergen-specific IgE results being reported by American clinical laboratories today.The HY-TEC-288 Plus and the Hycor Turbo-MP are automated assays based on the early paper disc RAST. The HY-TEC-288 Plus is highly automated and can report allergen-specific IgE results in kilo-international units per liter (kIU/L) that are interpolated from total serum IgE calibration curve. The Hycor Turbo-MP is a comparable assay system used by only one clinical laboratory that has the test volume sufficient to support its use. Both methods deserve the classification as “third-generation” methods but have not yet achieved this potential in the market place.One final point deserves comment. The organizers of the College of American Pathologists' SE survey are considering switching to the sole acceptance of quantitative kIU/L IgE antibody results and not accepting IgE antibody class results. Since there is now substantial scientific support for the clinical utility of quantitative IgE antibody results in the diagnosis of food and respiratory allergy,2Hamilton R.G. Adkinson Jr., N.F. In vitro assays for the diagnosis of IgE mediated disorders.J Allergy Clin Immunol. 2004; 114: 213-225Abstract Full Text Full Text PDF PubMed Scopus (150) Google Scholar this transition to quantitative IgE antibody results appears warranted. Our hope is that this transition will prompt more laboratories to make quantitative IgE antibody results available to their clients. To the Editor: Drs Kontis and Nayak1Kontis K.J. Nayak N. Hycor manufactures truly quantitative allergen-specific IgE assays.J Allergy Clin Immunol. 2005; 115: 639Abstract Full Text Full Text PDF PubMed Scopus (1) Google Scholar are correct in indicating that our review2Hamilton R.G. Adkinson Jr., N.F. In vitro assays for the diagnosis of IgE mediated disorders.J Allergy Clin Immunol. 2004; 114: 213-225Abstract Full Text Full Text PDF PubMed Scopus (150) Google Scholar did not highlight the Hycor (Hycor Stratagene, Garden Grove, Calif) IgE antibody assay methods as being automated and quantitative “third-generation assays.” Our conclusions were based on the lack of any quantitative IgE antibody data being reported for the Hycor assays to the College of American Pathologists' Diagnostic Allergy (SE) Survey. Since the SE survey is the most widely subscribed independent diagnostic allergy proficiency survey in North America, we believe that it accurately reflects the state of allergen-specific IgE results being reported by American clinical laboratories today. The HY-TEC-288 Plus and the Hycor Turbo-MP are automated assays based on the early paper disc RAST. The HY-TEC-288 Plus is highly automated and can report allergen-specific IgE results in kilo-international units per liter (kIU/L) that are interpolated from total serum IgE calibration curve. The Hycor Turbo-MP is a comparable assay system used by only one clinical laboratory that has the test volume sufficient to support its use. Both methods deserve the classification as “third-generation” methods but have not yet achieved this potential in the market place. One final point deserves comment. The organizers of the College of American Pathologists' SE survey are considering switching to the sole acceptance of quantitative kIU/L IgE antibody results and not accepting IgE antibody class results. Since there is now substantial scientific support for the clinical utility of quantitative IgE antibody results in the diagnosis of food and respiratory allergy,2Hamilton R.G. Adkinson Jr., N.F. In vitro assays for the diagnosis of IgE mediated disorders.J Allergy Clin Immunol. 2004; 114: 213-225Abstract Full Text Full Text PDF PubMed Scopus (150) Google Scholar this transition to quantitative IgE antibody results appears warranted. Our hope is that this transition will prompt more laboratories to make quantitative IgE antibody results available to their clients. Hycor manufactures truly quantitative allergen-specific IgE assaysJournal of Allergy and Clinical ImmunologyVol. 115Issue 3PreviewTo the Editor: Full-Text PDF
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