Reply to 'Mechanism for microtsunami-induced intercellular mechanosignalling'

Justin C Luo,Elliot L Botvinick,Vasan Venugopalan

doi:10.1038/nphoton.2015.165

Abstract

Reply to 'Mechanism for microtsunami-induced intercellular mechanosignalling'

Highlights

Luo et al reply — We thank He et al.[1] for their Correspondence regarding our recent paper[2]
They offer an alternate hypothesis for our observed microtsunamiinitiated Ca2+ signalling that differs from our claim of mechanical activation of stretch-sensitive G proteincoupled receptors (SS-GPCRs) by shear flow. They assert that μtsunami exposure initiates the release of Ca2+ signalling molecules, including ATP, from the cell immediately below the site of laser radiation. These molecules diffuse to neighbouring cells and activate purinergic receptors, including those coupled to GPCRs, resulting in a propagating intercellular Ca2+ wave
Because Ca2+ signalling was unaffected by PPADS but reduced by more than 70% with Reactive Blue 2 (RB2), the authors concluded that P2Y receptors play a central role in femtosecond laser induced signalling of astrocytes[5]

Summary

Introduction

Luo et al reply — We thank He et al.[1] for their Correspondence regarding our recent paper[2]. They offer an alternate hypothesis for our observed microtsunami (μtsunami)initiated Ca2+ signalling (μT-ICS) that differs from our claim of mechanical activation of stretch-sensitive G proteincoupled receptors (SS-GPCRs) by shear flow.

Results

Conclusion