Reply to Comment on "Differential Effects of MitoVitE, α-Tocopherol and Trolox on Oxidative Stress, Mitochondrial Function and Inflammatory Signalling Pathways in Endothelial Cells Cultured under Conditions Mimicking Sepsis. Antioxidants 2020, 9(3), 195".

Beverley E Minter,Nigel R Webster,Helen F Galley,Damon A Lowes

doi:10.3390/antiox9060464

Beverley E Minter, Nigel R Webster + Show 2 more

Open Access

https://doi.org/10.3390/antiox9060464

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Journal: Antioxidants	Publication Date: Jun 1, 2020
Citations: 4	License type: CC BY 4.0

Affiliation: University of Aberdeen

Abstract

We thank Drs Hegarty and Byrne for their interest in our paper and appreciate the opportunity to respond to their insightful comments [...].

Highlights

Hegarty and Byrne [1] state that Alamar Blue should not be used for the determination of ‘’mitochondrial function” when studying vitamin E, because Alamar Blue works via a redox-based mechanism
We find that profound mitochondrial membrane disruption occurs when MitoSOX red is used with antioxidants coupled to TPP such as MitoVitE or Mito Q
In our paper [3], we report a single concentration of the forms of vitamin E as proof of concept, as noted by Hegarty and Byrne

Summary

Introduction

1. Hegarty and Byrne [1] state that Alamar Blue should not be used for the determination of ‘’mitochondrial function” when studying vitamin E, because Alamar Blue works via a redox-based mechanism. As part of our preliminary assay development and optimization, we undertook a series of experiments in the presence of a range of antioxidants including various forms of vitamin E, both with and without LPS/PepG. We did not find that antioxidants affected the rate of resazurin reduction at the concentrations we tested.

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