Abstract
Previous studies from the Dalal and Henikoff groups compared atomic force microscopy (AFM) measurements of heights of nucleosomes containing histone H3 and CENP-A from chromatin arrays that had been extracted from human or Drosophila cells and enriched by immunoprecipitation1-4. In each study, CENP-A nucleosomes were observed to be lower in height than were H3 nucleosomes, and the authors concluded that CENP-A nucleosomes are tetrameric hemisomes with one copy of each histone; half the components of regular octameric H3 nucleosomes. To date, the suggestion that CENP-A nucleosomes are hemisomal in vivo remains heavily reliant on AFM data and has proven controversial because it conflicts with several in vitro and in vivo studies demonstrating that CENP-A nucleosomes contain CENP-A dimers and are octameric, as are all other known histone-variant nucleosomes5.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
