Abstract
Procedures for the isolation and in vitro maintenance of oval (periductular) cells obtained from pancreas of rats maintained on copper-deficient diet for 8 wk are described. Oval cells grew readily in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, and have been continuously propagated in culture for 6 mo. through 10 passages. Morphologically, these cells showed epithelial features characterized by frequent cell junctions, microvilli, intermediate filaments, and attempted lumen formation. These cells were negative for y-glutamyltranspeptidase but stained positively with OV-6 antibodies. Chromosomal analysis revealed hyperdiploid pattern with a model number of 68. These pancreatic oval cells of rat pancreas should serve as a valuable tool for establishing subcloned cell lines for the study of transdifferentiation and in establishing the stem-cell potential.
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