Abstract
The 3H-thymidine labelling patterns of isolated, enzyme-treated L cell metaphase chromosomes indicate that DNA synthesis occurs at numerous sites within each chromosomal fibre, and that each fibre contains many tandemly-joined replicating units. It is suggested that there may be clusters of adjacent replication units in the chromosome which undergo DNA synthesis relatively synchronously within the period of the 3H-thymidine pulse. The length of the chromosomal fibres was quite variable, but some fibres attained a length of over 1 mm.
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