Abstract
Replication origins in pea chloroplast DNA (ctDNA) have been mapped in the 44 kbp SalI fragment A. Restriction digests of pea ctDNA with SmaI, PstI and SalI/SmaI have allowed orientation of the two D-loops on the restriction map. One D-loop is located in the spacer region between the 16S and 23S rRNA genes, and the second D-loop is located downstream of the 23S rRNA gene, about 6 kbp apart from the first D-loop. Denaturation mapping of two recombinants, pCP12–7 and pCBI-12, which contain the two D-loops, confirmed the location of the D-loops. Denaturation mapping studies also showed that the D-loop present in the rRNA spacer region has a much higher A+T content than the other D-loop.
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