Abstract
The synthesis of viral DNA in diplophage-infected Streptococcus pneumoniae has been studied. A rapidly sedimenting complex was found in detergent lysates of bacteriophage Dp-4-infected cells by sedimentation in neutral sucrose gradients. The complex contained labeled parental viral DNA and newly synthesized DNA. Pulse-chase experiments demonstrated that fast-sedimenting material is metabolically active and serves as precursor for the formation of Dp-4 DNA found free in the cytoplasm and in mature phage particles. This intermediate incorporated most of the radioactivity during a short pulse of [ 3H]thymidine. The complex was dissociated by treatment with Pronase, sodium dodecyl sulfate, and NaOH. Parental DNA did not contribute to mature phage DNA. It is suggested that the fast-sedimenting material represents the replicating phage DNA specifically attached to some other constituent of the infected cell.
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