Abstract
The doubling time, virus yield, and susceptibility of five lepidopteran cell lines, Trichoplusia ni, Spodoptera frugiperda, Heliothis virescens, Plutella xylostella, and Anticarsia gemmatalis to the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) were compared. Isoelectric focusing provided isoenzyme patterns that specifically characterized each cell line. Cell doubling times ranged from 24 hr ( A. gemmatalis) to 30 hr ( H. virescens). Although the H. virescens cell line produced the highest viral titer (statistically equivalent to the T. ni cell line), the P. xylostella line produced the greatest number of polyhedral inclusion bodies (PIB). PIB from all five cell lines were virulent for T. ni larvae; PIB from the T. ni and H. virescens lines had the highest and lowest virulence, respectively.
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