Abstract
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM. The virus employed in this model is based on an engineered vector incapable of replication after initial infection. We show that despite this strategy, replication competent viral particles are released into the culture medium due to recombination between overlapping sequences of the env deleted HIV genome that is co-transfected with intact env. This finding emphasizes the need for careful data analysis and interpretation if similar constructs are employed and urges for additional caution during laboratory work.Electronic supplementary materialThe online version of this article (doi:10.1186/s12977-014-0070-3) contains supplementary material, which is available to authorized users.
Highlights
The central memory T cell (TCM) model forms a unique HIV-1 latency model based on primary cells that closely resemble in vivo TCM
To generate a vector capable of a single round of replication only, the vector employed in the original TCM model is produced with the env-deficient DHIV construct co-transfected with intact env
The positive signal in cells infected with DHIV + pLET-LAI indicates the presence of a full length env sequence in the viral DNA
Summary
Pawel Bonczkowski1†, Ward De Spiegelaere1†, Alberto Bosque, Cory H White, Anouk Van Nuffel, Eva Malatinkova, Maja Kiselinova, Wim Trypsteen, Wojciech Witkowski, Jolien Vermeire, Bruno Verhasselt, Laura Martins, Christopher H Woelk, Vicente Planelles and Linos Vandekerckhove1*
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