Abstract
The large (L) protein of vesicular stomatitis virus (VSV), catalytic subunit of RNA-dependent RNA polymerase is responsible for the transcription and replication of VSV. The L protein of the Indiana serotype of VSV (VSV Ind) has previously been cloned and expressed, and used in the reverse genetics of VSV Ind. However, the cDNA clones expressing functional L proteins of the VSV NJ serotype were not available. It was necessary to obtain functional clones of the New Jersey serotype of VSV (VSV NJ) in order to study homologous viral interference. Here we report the cDNA cloning, expression, and functional analyses of L proteins from both the Hazelhurst subtype and Concan subtype of VSV NJ. The analysis of the expressed L proteins for the transcription and replication of VSV demonstrate that both VSV NJ L clones express functional RNA-dependent RNA polymerase.
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