Abstract
The beta heavy chain subunit (β-MyHC) of cardiac myosin is the predominant isoform of human ventricular cardiomyocytes (CMs), whereas the human atrial myocardium contains a mixed population of α- and β-MyHC expressing CMs. We have previously shown that long-term culture of human embryonic and induced pluripotent stem cell-derived CMs (hESC-CMs, hiPSC-CMs) on laminin coated glass coverslips resulted in about 80% of CMs expressing exclusively β-MyHC.
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