Repetitive-Element PCR Technology for Identification of Mulberry Pathogens

Abstract

The coronatine is a new-type and high-efficient biogenic chlorosis-inducing phytotoxin. It’s also a new potential chemical regulator. Plants can get higher stress and disease resistance with spraying coronatine at a low concentration, which has broad application prospects. It’s an effective way to ferment coronatine industrially using Pseudomonas syringae. The REP universal primers were used to establish the repetitive sequence PCR technology. Clustering analysis and classification were used to screen the wild strains producing coronatine from Jiangsu and Zhejiang area. The results showed that 29 strains were divided into five groups with similarity coefficient of 0.65. The cluster analysis showed the highest diversity of P. syringae pv. mori appeared in mulberry organization form Chun-An, which is relevant with the regional water conditions. It provides a new thought to screen the wild strains producing coronatine.