Repertoire of noncoding RNAs in corpus luteum of early pregnancy in buffalo (Bubalus bubalis).

S M K Thirumaran,A Jerome,S N Kala

doi:10.14202/vetworld.2017.1129-1134

Abstract

Aim:The present study was designed to identify other noncoding RNAs (ncRNAs) in the corpus luteum (CL) during early pregnancy in buffalo.Materials and Methods:For this study, CL (n=2) from two buffalo gravid uteri, obtained from the slaughter house, was transported to laboratory after snap freezing in liquid nitrogen (−196°C). The stage of pregnancy was determined by measuring the crown-rump region of the fetus. This was followed by isolation of RNA and deep sequencing. Post-deep sequencing, the obtained reads were checked and aligned against various ncRNA databases (GtRNA, RFAM, and deep guide). Various parameters, namely, frequency of specific ncRNAs, length, mismatch, and genomic location target in several model species were deciphered.Results:Frequency of piwi-interacting RNAs (piwi-RNAs), having target location in rodents and human genomes, were significantly higher compared to other piwi-RNAs and ncRNAs. Ribosomal RNAs (rRNAs) deduced had nucleotides (nts) ranging from 17 to 50 nts, but the occurrence of small length rRNAs was more than lengthier fragments. The target on 16S rRNA species confirms the conservation of 16S rRNA across species. With respect to transfer RNA (tRNA), the abundantly occurring tRNAs were unique with no duplication. Small nucleolar RNAs (snoRNAs), identified in this study, showed a strong tendency for coding box C/D snoRNAs in comparison to H/ACA snoRNAs. Regulatory and evolutionary implications of these identified ncRNAs are yet to be delineated in many species, including buffaloes.Conclusion:This is the first report of identification of other ncRNAs in CL of early pregnancy in buffalo.

Highlights

Corpus luteum (CL), a dynamic ovarian endocrine structure, through its sequential morphological and functional changes, helps in the production and secretion of progesterone
With respect to transfer RNA, the abundantly occurring tRNAs were unique with no duplication
Regulatory and evolutionary implications of these identified ncRNAs are yet to be delineated in many species, including buffaloes

Summary

Introduction

Corpus luteum (CL), a dynamic ovarian endocrine structure, through its sequential morphological and functional changes, helps in the production and secretion of progesterone. This release of progesterone is imperative for controlling estrus cycle along with its priming effect for estrus behavior and maintenance of pregnancy [1]. The synchronized functioning of CL is controlled by many paracrine, endocrine factors and genes during the estrous cycle and pregnancy [2]. Noncoding RNAs (ncRNAs) have been identified in varied biological tissues, including CL [4]. MicroRNAs (miRNAs) are noncoding single-strand RNAs, 17-22 nts in length, that play a role in the regulation of gene expression

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