Repeating use of Chalara paradoxa amylase by α‐cyclodextrin affinity column

Abstract

much attention as an energy saving technology for starch saccharification or alcohol fermentation. It is applicable to immobilize raw starch-digesting amylase for repeating use. As it is difficult to hydrolyze solid raw starch with solid immobilized enzymes, the immobilizations to special carriers or membranes of raw starch-digesting glucoamylases have been reported. Hayashida and Florl reported that the raw starch-digesting glucoamylase I of Aspergillus awamori var. kawachi was tightly bound onto chitin without the aid of a cross linking reagent. Matsumoto and Kunisaki2 reported that the glucoamylases were adsorbable onto glucomannan and the immobilized glucoamylases enabled to digest raw starch. Matsumura and co-workers3 reported that the simultaneous saccharification and fermentation of raw starch were tested in a membrane reactor. In previous reports ,4-6 Kainuma and co-workers reported the discovery of a new raw starch-digesting amylase from Chalara parudoxa. Monma et aL7 reported the preparation of Chalara paradoxa glucoamylase by a-cyclodextrin Sepharose 6B affinity column chromatography efficiently.