Abstract
Repeated batch fermentation is a stable, economically viable and environment friendly fermentation system. Biofilm of Bacillus subtilis was investigated for repeated batch fermentation for the production of protease enzyme. It was found that complete replacement of spent medium with fresh medium supplementation in biofilm produced similar amount of protease that is about 225 U/ml of enzyme in successive batches. Moreover, unlike some solid carriers the EPS layer does not entrap much enzyme inside it. Therefore, instead of using immobilized cells, biofilm of B. subtilis can be a good candidate for protease enzyme production using repeated batch fermentation.Jahangirnagar University J. Biol. Sci. 6(2): 29-38, 2017 (December)
Highlights
For production of various secondary metabolites, several fermentation processes are being used to run a stable, economically viable and environment friendly system
Immobilized microbial cells are frequently applied for bioconversion, biotransformation and biosynthesis processes due to their better operational stability, easier separation from products for possible reuse, and satisfactory efficiency of catalysis as compared to free cells (Kroutil et al, 1998; Pakula & Freeman, 1996; Gill & Ballesteros, 2000)
Cells and enzymes seem to be protected by matrices (Liu, 1998; Jirku, 1999; Junter et al, 2002) and physiology of immobilized viable cells can be favorably modified in comparison to free cells (Abdel-Naby et al, 2000; Angelova et al, 2000; Junter et al, 2002)
Summary
For production of various secondary metabolites, several fermentation processes are being used to run a stable, economically viable and environment friendly system. Repeated batch is one of the fermentation procedures that is reportedly useful and advantageous over other fermentation systems (Abdel-Naby et al, 2000; Carmichael et al, 2000). In repeated batch fermentation microbial cells are reused for subsequent fermentation runs. For the reuse of microbial cells, the cells usually need to be immobilized in solid carriers. Immobilized microbial cells are frequently applied for bioconversion, biotransformation and biosynthesis processes due to their better operational stability, easier separation from products for possible reuse, and satisfactory efficiency of catalysis as compared to free cells (Kroutil et al, 1998; Pakula & Freeman, 1996; Gill & Ballesteros, 2000). One of the biggest drawbacks of addition of solid carriers is their limited feasibility for industrial use. Degradation of the carrier material creates much complexity during product separation and purification (Xiu et al, 2000)
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