Abstract
Abstract— Ultraviolet inactivation ofHaemophilus influenzae transforming DNA followed inverse square root kinetics in both mismatch repair-proficient(hex+) and deficient(hex-1) recipients. No DNA concentration effect was seen with UV-excision repair-deficient(uvr-) strains. Low-efficiency genetic markers remained more sensitive than high-efficiency ones when they were assayed on excision repair-deficienthex+ uvr- strains. They were equally resistant whenhex+ uvr- recipients were used. We explain this by assuming that recombinational repair of UV lesions in the donor strand and mismatch repair of the recipient strand may overlap and cause double strand interruptions. This will eliminate low-efficiency transformants.
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