Repair of rabbit's radius segmental bone defect with sintered bovine cancellous bone complexed with a BMP2-derived peptide and collagen I

Abstract

Objective To observe the repair of bone defect with sintered bovine cancellous bone complexed with synthetized BMP2-derived peptide and collagen Ⅰ.Methods Rabbits were divided into 3 groups.Sintered bovine bone was implanted in group A.Sintered bovine bone complexed with collagen 1 was,implanted in group B.Sintered bovine bone complexed with BMP2-derived peptide/eollagen 1 was implanted in group C.Before implantation,the materials were observed under the environmental scanning electron microscopy(ESEM).Thirty-six rabbits were gained to establish models of 10-mm middle radius bone defects and divided into 3 groups with 12 in each group.Postoperatively.the formed new bone was compared among the three implants at the 4th,8th,12th week by X ray score,and at the 8th,12th week by histological score.The bend intensity of implanted materials at the 12th week were tested.respectively.Resuits Thirty-six rabbits were all involved in the resuh analysis.(1)ESEM revealed that the porosity of compound materials was high with close compound.(2)At different postoperative time points,the X ray scores on the group C were significantly greater than in groups A and B(P＜0.05).(3)The histological scores at the 4th and 8th week on the group C were 6.83±0.41 abnd 8.67±0.52 respectively.significanfly higher than in both group A(4.67±0.52,6.17±0.41)and group B(5.33±0.52,7.33±0.52)respectively(P＜0.05).(4)The bend intensity of implanted materials in group C at the 12th week after operation was higher significantly than that in groups A and B.Conclusion Sintered bovine cancellous bone and its compound materials can repair the bone defect,but the sintered bovine bone complexed with BMP2-derived peptide/collagen I is the most ideal. Key words: Sintered bone; Bone defect; BMP2; Type Ⅰ collagen