Abstract
Objective To assess the effect of bone defect repair using the recombinant of adenovirus-mediated hBMP2 and hVEGF165 genes transfer of BMSCs with porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66). Methods Sixty male adult New Zealand rabbits were assigned to groups A, B and C according the completely random design, with 20 rabbits per group. Bone defect of 15 mm in length was made in the middle segment of bilateral radii in rabbits. In Group A, the defects were filled with nothing on the left side in blank controls (Group A1) and with n-HA/PA66 material alone on the right side (Group A2). In Group B, the defects were filled with hVEGF165/BMSCs/n-HA/PA66 on the left side (Group B1) and hBMP2/ BMSCs/n-HA/PA66 on the right side (Group B2). In Group C, the defects were filled with BMSCs/n-HA/PA66 on the left side (Group C1) and hBMP2/hVEGF165/BMSCs/n-HA/PA66 on the right side (Group C2). Radiological analysis, HE staining, and Masson coloration were performed 2, 4, 8 and 12 weeks after operation. Results Radiographs, HE staining and Masson staining taken 8 weeks after cell transplantation showed large amount of new cartilage grown into the defect area and massive bony tissue formation around the margin in Group C2. At postoperative 12 weeks, Group C2 showed transplants were surrounded by outer bone tissues with superior bone repair effect to other groups (P 0.05). Conclusion hBMP2/hVEGF165 genes transferred BMSCs seeded on porous n-HA/PA66 can contribute to osteogenesis during the repair of rabbit radius defect. Key words: Bone morphogenetic proteins; Vascular endothelial growth factors; Bone defect
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.