Abstract
Oxidative stress has been identified as an underlying factor in the development of insulin resistance, β-cell dysfunction, impaired glucose tolerance and type 2 diabetes mellitus and it also play major role in kidney stone formation. The present study is aimed to elucidate the in vitro nephroprotective activity of two isoflavonoid glycosides, tectorigenin 7-O-β-D-glucosyl-(1→6)-β-D-glucoside (1) and tectorigenin 7-O-β-D-glucosyl-4′-O-β-D-glucoside (2) isolated from the n-BuOH fraction of Iris spuria L. (Zeal) rhizome MeOH extract against oxalate and high glucose-induced oxidative stress in NRK-49F cells. The results revealed that compounds 1 and 2 significantly increased the antioxidant enzyme activities and decreased MDA levels in both oxalate and high glucose stress. Treatment with these phytochemicals effectively down-regulated expression of crystal modulator genes and pro-fibrotic genes in oxalate and high glucose-mediated stress respectively. This study indicates cytoprotective, antioxidant, anti-urolithic and anti-diabetic effects of compounds 1 and 2 against oxalate and high glucose stress.
Highlights
Oxidative stress is linked to many pathological disorders and associated with elevated intracellular levels of reactive oxygen species (ROS) that cause damage to lipids, proteins and DNA
Chromatographic separation of the n-BuOH fraction obtained from the 70% MeOH extract of I. spuria rhizomes resulted in the isolation of 2 isoflavonoid glycosides
The results showed that compounds tectorigenin 7-O-b-D-glucosyl-(1!6)-b-D-glucoside (1) and tectorigenin 7-O-b-D-glucosyl-40-O-b-Dglucoside (2) protected the renal fibroblasts against both oxalate and high glucose induced oxidative stress by increasing cell viability
Summary
Oxidative stress is linked to many pathological disorders and associated with elevated intracellular levels of reactive oxygen species (ROS) that cause damage to lipids, proteins and DNA. Novel therapeutic approach targeting oxidative stress and fibrosis for both kidney stone disease and diabetes associated renal damage is necessary. O-b-D-glucoside (2) obtained from the n-BuOH fraction of I. spuria rhizome MeOH extract against oxalate and high glucose-induced oxidative stress in NRK-49F cells.
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