RENOPROTECTIVE ACTION OF PHYTOTHERAPY IN EXPERIMENTAL METABOLIC SYNDROME

Abstract

Purpose. To determine the efficacy of the therapeutic and prophylactic effect of two phytopreparations on the kidneys of rats with the metabolic syndrome. Material and methods. We used the “Grape” extract (water-alcohol extract from the grape squeeze) and the extract “Oak” containing phenolic substances from oak. The experiments were performed on rats that reproduced metabolic syndrome (MS) by keeping rats on a high-fat diet, while reproducing intestinal dysbiosis and immunodeficiency (due to the introduction of cytostatics). The “Grape” extract was administered with food, and the “Oak” extract with drinking water, both at a dose of 50 mg of dry matter per kg of live weight. Herbal preparations were administered daily for 40 days. The activity of elastase, urease, lysozyme and ALT was determined in the blood serum. In the homogenate of the kidneys, the activity of elastase, urease, lysozyme, catalase and the content of MDA were determined. The antioxidant-prooxidant API index was calculated by the ratio of catalase activity and MDA content, and the degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme. Results. It has been shown that in rats with MS in the kidneys, the activity of elastase, urease, the content of MDA and the degree of dysbiosis increase with a decrease in the activity of lysozyme, catalase and the API index. In the blood serum of rats with MS, the activity of elastase, urease, ALT increases and the activity of lysozyme decreases. Phytopreparations decreased elastase, urease activity, MDA content and the degree of dysbiosis, but increased lysozyme activity, catalase and the API index; these effects were more pronounced in the Oak extract. Conclusions. In kidney under MS, an inflammatory-dystrophic process develops due to systemic inflammation and bacteremia. Phytopreparations, especially Oak, have a renoprotective effect.