Renalase rs10887800 gene polymorphism and its serum level in preeclampsia

Abstract

A Preeclampsia (PE) is the commonest dangerous disorder during pregnancy and preeclampsia is characterized by increased level of (epinephrine and nor epinephrine). Renalase is an enzyme that cause break down of catecholamines, that leads to elevation of blood pressure and incidence of PE. This work aims to study the relation between renalase (RNLS rs10887800) polymorphism and its serum level with susceptibility to PE.This study was carried out on 150 subjects.One hundred of them were patients with preeclampsia (fifty with severe Preeclampsia (group I) and fifty with mild preeclampsia (group II) who were attendants and inpatients in Obstetrics and Gynecology Department of Menoufia University hospital. Fifty subjects included in this study were women with normal pregnancy as a control group (groupIII). The patients and the control groups were age matched. Serum renalase was measured by using enzyme-linked immunosorbent assay (ELISA) technique And Renalase rs10887800 (A/G) polymorphism was analyzed using the TaqMan allelic discrimination assay technique (real time PCR). Results showed: a significant statistical differences between the studied groups regarding serum renalase level and Renalase rs10887800 (A/G)genotype distribution with decreased serum renalase level and increased frequency of the GG genotype and G allele was observed in patients with Preeclampsia more than in control. Conclusion: GG genotypes of Renalase rs10887800 (A/G)gene polymorphism and its associated low serum renalase level could be a genetic risk factor for PE.