Renal Oxidized Fatty Acids are Reduced in LDL Receptor Deficient Mice following treatment with Anti Inflammatory Peptide F

Abstract

IntroductionLDL receptor deficient mice have high levels of apoB containing lipoproteins that upon feeding the animals with a Western Type diet, further increase in concentration and bring about lipid oxidation, inflammatory and acute phase response. Oxidized fatty acids such as HETEs and HODES are known to induce inflammatory molecules in various organs and result in metabolic complications.ObjectiveIn the present study we sought to determine the effect of a Western type Diet on renal content of oxidized fatty acids and the potential beneficial effect of the anti inflammatory peptide 4F.MethodsFemale LDL R−/− mice (n=20 per treatment group) were maintained on a Western Diet for 6 weeks and were given placebo or anti inflammatory peptide 4F in the drinking water supplying 0.9 mg per mouse per day. Kidneys were analyzed for HETEs and HODEs using LC‐ESI‐MS/MS.ResultsThe concentration of 9‐HODE, 13‐HODE, 5‐HETE, 12‐HETE, 15‐HETE, 20 HETE 14, 15EET and arachidonic acid itself were significantly lower in the group that received the peptide as compared to the mice that did not receive additional peptide (p from 0.001 to 0.042).ConclusionThe antiinflammatory peptide 4F is capable of interfering with the oxidative cascade, sequestering oxidized fatty acids and reducing their concentration in the organism including in the kidneys. The present findings might have major implications in prevention of renal disorders in hyperlipemia.