Renal overflow of noradrenaline and dopamine to plasma during hindquarter compression and thoracic inferior vena cava obstruction in the dog.

Abstract

Efferent renal nerve activity was assessed by measurements of the overflow of endogenous noradrenaline (NA) and dopamine (DA) to plasma in the kidney. To obtain correct estimates of the renal contribution to the renal venous outflow of NA and DA, corrections for the renal extraction of catecholamines in arterial plasma were performed by use of tracer amounts of [3H]NA. Hindquarter compression (previously known to cause a neurogenically mediated blood pressure elevation) increased the concentrations of NA, adrenaline and DA in arterial and renal venous plasma. The renal overflow of NA increased from 83.7 +/- 32.0 to 361.3 +/- 119.4 pmol min-1 (P less than 0.05) during hindquarter compression. When compared to the renal NA overflow during electrical renal nerve stimulation, this corresponds to an increase in average renal nerve impulse activity from approximately 0.4 to 1.6 Hz. Hindquarter compression also increased the renal overflow of DA to plasma. When venous return to the heart was reduced by obstruction of the thoracic inferior vena cava, the mean arterial blood pressure fell and all catecholamines in plasma increased gradually during the first 10 min of obstruction. The renal overflow of NA was only slightly increased, indicating a minor increase in renal nerve activity. The overflow of DA to plasma was not altered by obstruction of the thoracic inferior vena cava. Neither maneuver substantially altered the DA/NA ratio for renal overflow rates or for renal venous plasma concentrations indicating that there was no preferential activation of either noradrenergic or putative dopaminergic nerve fibres.