Renal Clearable Gold Nanoparticle-Functionalized Silk Film for in vivo Fluorescent Temperature Mapping.

Wei Hua,Daniel Boyer,Shengyang Yang,Yusheng Mao,Guolin Zhang,Shengling Lin,Jinzhu Zhang,Shasha Sun,Lang Liu,Fenfen Zheng,Chen Zhou

doi:10.3389/fchem.2020.00364

Abstract

Implantable optical sensing devices that can continuously monitor physiological temperature changes hold great potential toward applications in healthcare and medical field. Here, we present a conceptual foundation for the design of biocompatible temperature sensing device by integrating renal clearable luminescent gold nanoparticles (AuNPs) with silk film (AuNPs-SF). We found that the AuNPs display strong temperature dependence in both near-IR fluorescence intensity and lifetime over a large temperature range (10–60°C), with a fluorescence intensity sensitivity of 1.72%/°C and lifetime sensitivity of 0.09 μs/°C. When integrated, the AuNPs with biocompatible silk film are implanted in the dorsal region of mice. The fluorescence imaging of the AuNPs-SF in the body shows a linear relationship between the average fluorescence intensity and temperature. More importantly, <3.68% ID gold are left in the body, and no adverse effect is observed for 8 weeks. This AuNPs-SF can be potentially used as a flexible, biocompatible, and implantable sensing device for in vivo temperature mapping.

Highlights

Implantable optical sensing devices (Dong et al, 2012; Ruckh and Clark, 2014) that are capable of reporting physiological information offer great opportunities for biomedical diagnosis, monitoring, and therapy (Balaconis and Clark, 2012; Brites et al, 2012)
Fluorescence-based sensing devices are of great interest due to their high spatial and temporal resolution, fast response, and ability to work in strong electromagnetic fields (Li et al, 2019)
A large amount of research efforts have been devoted to hybrid inorganic fluorescent nanosensors such as carbon dots (Chandra and Singh, 2017; Khan et al, 2018), quantum dots (Zheng et al, 2015; Du et al, 2018), and gold nanoparticles (AuNPs) (Carattino et al, 2018; Fatino et al, 2018; Ma et al, 2018; Zhao et al, 2019) with biocompatible polymers (Zhou et al, 2009; Sun et al, 2016; Wu et al, 2019), hydrogel (Ming et al, 2016), or silk (Cheng et al, 2018) for implantable fluorescent sensing devices

Summary

INTRODUCTION

Implantable optical sensing devices (Dong et al, 2012; Ruckh and Clark, 2014) that are capable of reporting physiological information offer great opportunities for biomedical diagnosis, monitoring, and therapy (Balaconis and Clark, 2012; Brites et al, 2012). Fluorescence-based sensing devices are of great interest due to their high spatial and temporal resolution, fast response, and ability to work in strong electromagnetic fields (Li et al, 2019) In this context, a large amount of research efforts have been devoted to hybrid inorganic fluorescent nanosensors such as carbon dots (Chandra and Singh, 2017; Khan et al, 2018), quantum dots (Zheng et al, 2015; Du et al, 2018), and gold nanoparticles (AuNPs) (Carattino et al, 2018; Fatino et al, 2018; Ma et al, 2018; Zhao et al, 2019) with biocompatible polymers (Zhou et al, 2009; Sun et al, 2016; Wu et al, 2019), hydrogel (Ming et al, 2016), or silk (Cheng et al, 2018) for implantable fluorescent sensing devices. These results indicate that the AuNPs-SF is promising to serve as implantable and wearable temperature sensing devices due to its superior sensitivity, biocompatibility, and processability

MATERIALS AND METHODS

Ethics Statement

AND DISCUSSION

Findings

CONCLUSIONS