Abstract
SUMMARY:Aim: We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus.Methods: Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP‐transgenic rats followed by delivery of electric pulses.Results: The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells.Conclusion: Electroporation‐mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step‐by‐step description of this perfusion method and a discussion of the key points for the success of the technique.
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