Abstract
Abstract The biochemical assay of alkaline phosphatase (EC 3.1.3.1) activity in the kidney of the adult Triturus cristatus carnifex is reported. In normal animals the activity of renal alkaline phosphatase was notably depressed in the terrestrial compared with the aquatic phase. In both phases arginine-vasotocin (AVT) elicited a marked reduction in the enzyme activity. Exogenous prolactin (PRL) induced no change in activity when administered to terrestrial animals. which are known to have a large store of endogenous PRL in the hypophysis, but low levels in blood. Hypophysectomy on aquatic animals markedly depressed renal alkaline phosphatase, but PRL administration brought back to normal the values of enzymatic activity. Thus it would appear that renal alkaline phosphatase is involved in osmoregulatory processes in the adult Triturus cristatus.
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