Removing artifacts in Second Harmonic Generation imaging by interferometry

Maxime Pinsard,Margaux Schmeltz,Jarno N Van Der Kolk,Francois Legare,Kessen Patten,Marie-Claire Schanne-Klein,Lora Ramunno

doi:10.1109/pn.2019.8819578

Removing artifacts in Second Harmonic Generation imaging by interferometry

Maxime Pinsard, Margaux Schmeltz + Show 5 more

https://doi.org/10.1109/pn.2019.8819578

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Publication Date: May 1, 2019

Affiliation: Institut National de la Recherche Scientifique, Inserm, French National Centre for Scientific Research, École Polytechnique, Institut Polytechnique de Paris, University of Ottawa, Armand Frappier Museum

#Interferometric Second Harmonic Generation #Regions Of Opposite Polarities + Show 8 more

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Abstract

We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.

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