Removal Performance of Antibiotic Resistance Genes and Heavy Metal Resistance Genes in Municipal Wastewater by Magnetic-Coagulation Process

Abstract

Antibiotic resistance genes (ARGs) in municipal wastewater pose a potential threat to the environment. In this study, the change in absolute and relative abundance of ARGs, metal resistance genes (MRGs), and mobile genetic elements (MGTs) were investigated during an emergent municipal wastewater treatment by the magnetic separation process. Results indicate that all the concentrations of targeted ARGs, MRGs, and MGTs decreased significantly in the primary and secondary stirring tank. However, the absolute abundance of some ARGs and MRGs increased in the effluent, which is likely caused by the presence of ample MGTs, in the order of int1 (2.00×1010 copies·mL-1) > int2 (1.91×108 copies·mL-1) > Tn 916/1545e(5.38×108 copies·mL-1). The results obtained from network and PCA analysis showed that the removal of ARGs and MRGs were significantly associated with variations in the microbial community and common pollutants in urban wastewater, such as suspended solids, phosphorus, and COD, which are important factors for affecting the removal efficiency of antibiotic resistance genes and metal heavy resistance genes. These results show that magnetic separation can effectively reduce common pollutants in urban wastewater and might further restrict the transmission and transfer of ARGs. Moreover, it is necessary to strengthen the subsequent management of magnetic separation effluent and dehydrated sludge by disinfection technologies to lessen the risk of antimicrobial contamination.