Abstract

The strain TZ-1 was screened from five isolates from soil sample of oil shale exploration area in Maoming by comparing the data of zinc removal percentage at pH 7.0 and 30 °C with initial Zn2+ concentration of 2 mmol/L in nutrient broth and MICs. The isolate TZ-1 was identified as Burkholderia sp. based on its morphological, physiological-biochemical characteristics and the 16S rDNA sequence. The initial Zn2+ concentration of 16 mmol/L in nutrient broth inhibited the growth of TZ-1 obviously. The maximum zinc removal percentage of TZ-1 was up to 67.83% with the initial zinc concentration of 8 mmol/L in the broth. The results of heavy metals affecting Zn2+ adsorption indicated that Zn2+ removal was increased when other heavy metals existed in the broth. The surface characterization of the adsorbent was done through FTIR. The results suggest that the hydroxyl, carboxyl, amine and phosphate groups of biomass surface play a vital role in Zn2+ adsorption. The biosorption process conforms Langmuir and Freundlich isotherms and can be described by the pseudo-second-order kinetic model. The data of application in wastewater treatment indicate that the strain TZ-1 is suitable for wastewater purification.

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