Abstract
ABSTRACT Our objective was to determine the effects of acute nutrient restriction on secretion of luteinizing hormone (LH) in serum of male cattle. Angus × Hereford steers (402 ± 17 kg) with rumen cannulas were randomly assigned to treatment. Control (n = 4) steers had ruminal contents completely removed and immediately replaced, whereas restricted (n = 4) steers had ruminal contents completely removed and only 15% (5 L) of rumen contents replaced. Beginning at 1600 h on d 0, control steers were fed 9 kg of prairie hay and 1 kg of a grain supplement each day, whereas restricted steers were fed 1.8 kg of prairie hay daily. Blood samples were collected twice daily for determination of plasma concentrations of glucose, nonesterified fatty acids, and urea nitrogen. Serial blood samples were collected at 10-min intervals for 8 h on d − 1, 0, 1, and 3 to determine serum concentrations of LH. Concentrations of LH (P
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