Abstract
The removal of an azo dye, namely Remazol Blue, by wastewater-isolated bacteria (Bacillus megaterium, Micrococcus luteus and Bacillus pumilus) has been investigated in media containing molasses as a carbon and energy source. The dye removal properties of bacteria were studied in a batch system where the initial pH values (6, 7, 8, 9) and dye concentrations are the variables (28.7, 57.9, 78.3, 97.9mg/L) throughout three days of incubation time. Optimal pH value was 7 for the tested bacteria in media containing 25mg/L dye. All of the isolated bacteria had capacity for removing Remazol Blue in the range from 23.7% to 69.9%. Moreover, experiments reveal notable results even 97.9mg/L dye concentrations in a short incubation time such as 3 days in the liquid media that contains only molasses as a carbon and energy source. The highest azoreductase activity was obtained as 39.9U/mL for B. pumilus at 2nd day while M. luteus did not show any azoreductase activity.
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